Biosci™ Lymphocyte Separation Tube for Human Peripheral Blood
The number of mononuclear cells (lymphocytes and monocytes) in peripheral blood is between 1.075-1.090 g/ml, which differs from that of red blood cells, multinuclear leukocytes, and platelets. The densities of red blood cells and granulocytes are relatively high (about 1.092 g/ml) and the density of platelets is between 1.030 and 1.035 g/ml.
Therefore, Human Lymphocyte Separation Medium is used to create a density gradient and the diluted whole blood is carefully layered on the separation medium. After centrifugation, the red blood cells and granulocytes settle at the bottom of the tube due to their high density. Since their densities are less than or equal to the density of the separation medium, lymphocytes and monocytes are found at the interface of the separation medium. There may also be a small amount of cells suspended within the medium. By aspirating the cells at the interface, mononuclear cells can be separated from peripheral blood.
This range of products offers several advantages such as fast and simple use, no cytotoxicity, high cell yield, and good vitality.
Integrated Sieve Plate for Direct Blood Loading
To streamline the process, a polyethylene sieve plate with large pores can be incorporated into the centrifuge tube. This allows whole blood to be directly added to the tube, eliminating the need for pre-mixing with separation medium below the sieve. This innovative design effectively minimizes the potential for red blood cell and granulocyte contamination.
The isolated cells can then be effortlessly poured directly from the tube.
Product Features:
- Ready-to-Use Convenience: Pre-filled separation medium eliminates manual preparation steps, saving time and reducing errors. Simply add anticoagulated blood directly to the tube without dilution.
- Enhanced Safety: Medical-grade, low-endotoxin separation medium protects the integrity and viability of isolated cells. Additionally, the non-toxic polypropylene tube and medical-grade sieve plate promote a safe and reliable workflow.
- Exceptional Separation Performance: Clear and distinct cell layers provide easy identification and retrieval of isolated cells. This results in high-purity and high-yield isolated cells, suitable for downstream applications.
Intended Use:
High-performance density gradient centrifugation for isolation of various biomolecules based on size and density.
Features:
- Simple and streamlined protocol minimizes processing time.
- Low cytotoxicity ensures high post-separation cell viability.
- Exceptional purity and yield for downstream applications.
- ISO 13485:2016 compliant for quality assurance.
- Superior clarity with minimal background interference for optimal sample visualization.
Intended Use:
Isolates human peripheral blood lymphocytes (PBMCs) and mononuclear cells (MNCs) from most mammals via density gradient centrifugation.
Features:
- Simplified Workflow: Streamlined protocol for efficient separation.
- Enhanced Biocompatibility: Sterile and low cytotoxicity for high cell viability.
- Superior Performance:
- Achieves high purity and yield of lymphocytes/MNCs.
- Well-defined separation layers for easy harvesting.
Intended use:
Enables downstream analysis of individual cells by generating a homogenous single-cell suspension.
Features:
- Ready-to-use solution for density-based cell separation from single-cell suspensions.
- Adjustable density for isolating various animal cell types.
- Biocompatible and chemically inert formulation ensures gentle separation.
- Superior Performance:
- Streamlined workflow with pre-mixed solution.
- High separation purity.
- Maintains cell viability.
Intended Use:
The BioSci Lymphocyte Separation Kit simplifies the isolation of lymphocytes from human peripheral whole blood. It utilizes a two-part system:
- BioSci Density Separation Media (Type A): This sterile, low-toxicity density gradient medium facilitates the separation of lymphocytes from other blood components based on their differing densities.
- BioSci Cell Separation Tubes (50mL): These medical-grade tubes are free of cytotoxicity and tissue toxicity, ensuring sample integrity during centrifugation.
Features:
- Fast and Easy: The kit provides a quick and convenient method for lymphocyte isolation.
- Safe and Reliable: The sterile, low-toxic media and medical-grade tubes minimize risks and ensure sample safety.
- High-Quality Results: The kit consistently delivers high cell viability, purity, and yield, meeting the requirements of ISO 13485:2016.
Intended Use:
The BioSci Cell Separation Kit offers versatile isolation of various cell types from small animal models. It enables the separation of:
- Lymphocytes from mouse or rat spleens
- Lymphocytes from mouse, rat, or rabbit blood
- Mouse bone marrow cells
Features:
- Simple and Efficient: The kit provides a user-friendly protocol for streamlined cell isolation.
- Safe and Biocompatible: The chemically inert and biocompatible formulation minimizes risks of cell damage or contamination.
- High-Quality Results: The kit consistently delivers high cell viability, purity, and yield for reliable downstream applications.